Cell News // 02 // 2013 - page 24

Gohl, C., Banovic, D., Grevelhorster, A., and Bogdan, S. WAVE forms hetero- and homo-oligomeric
complexes at integrin junctions in Drosophila visualized by bimolecular fuorescence comple-
mentation. J Biol Chem 285, 40171-40179.
Gustafsson, M. G., Shao, L., Carlton, P. M., Wang, C. J., Golubovskaya, I. N., Cande, W. Z., Agard,
D. A. & Sedat, J. W. (2008) Three-dimensional resolution doubling in wide-feld fuorescence
microscopy by structured illumination. Biophys J, 94, 4957-4970.
He, L., Wang, X. & Montell, D. J. (2011) Shining light on Drosophila oogenesis: live imaging of egg
development. Curr Opin Genet Dev, 21, 612-619.
Hudson, A. M. & Cooley, L. (2002a) A subset of dynamic actin rearrangements in Drosophila
requires the Arp2/3 complex. J Cell Biol, 156, 677-687.
Hudson, A. M. & Cooley, L. (2002b) Understanding the function of actin-binding proteins through
genetic analysis of Drosophila oogenesis. Annu Rev Genet, 36, 455-488.
Insall, R.H., and Machesky, L.M. (2009). Actin dynamics at the leading edge: from simple machi-
nery to complex networks. Dev Cell 17, 310-322.
Kunda, P., Craig, G., Dominguez, V. & Baum, B. (2003) Abi, Sra1, and Kette control the stability
and localization of SCAR/WAVE to regulate the formation of actin-based protrusions. Curr Biol,
13, 1867-1875.
Lebensohn, A. M. & Kirschner, M. W. (2009) Activation of the WAVE complex by coincident
signals controls actin assembly. Mol Cell, 36, 512-524.
Leibfried, A., Fricke, R., Morgan, M.J., Bogdan, S., and Bellaiche, Y. (2008). Drosophila Cip4 and
WASp defne a branch of the Cdc42-Par6-aPKC pathway regulating E-cadherin endocytosis. Curr
Biol 18, 1639-1648.
Linardopoulou, E.V., Parghi, S.S., Friedman, C., Osborn, G.E., Parkhurst, S.M., and Trask, B.J. (2007).
Human subtelomeric WASH genes encode a new subclass of the WASP family. PLoS Genet 3,
e237.
Liu, R., Abreu-Blanco, M.T., Barry, K.C., Linardopoulou, E.V., Osborn, G.E., and Parkhurst, S.M.
(2009). Wash functions downstream of Rho and links linear and branched actin nucleation fac-
tors. Development 136, 2849-2860.
Miki, H., Miura, K., and Takenawa, T. (1996). N-WASP, a novel actin-depolymerizing protein,
regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of
tyrosine kinases. Embo J 15, 5326-5335.
Miki, H., Suetsugu, S., and Takenawa, T. (1998). WAVE, a novel WASP-family protein involved in
actin reorganization induced by Rac. Embo J 17, 6932-6941.
Mullins, R. D., Heuser, J. A. & Pollard, T. D. (1998) The interaction of Arp2/3 complex with actin:
nucleation, high affnity pointed end capping, and formation of branching networks of flaments.
Proc Natl Acad Sci U S A, 95, 6181-6186.
Pollitt, A.Y., and Insall, R.H. (2009). WASP and SCAR/WAVE proteins: the drivers of actin assembly.
J Cell Sci 122, 2575-2578.
Pollard, T. D. & Borisy, G. G. (2003) Cellular motility driven by assembly and disassembly of actin
flaments. Cell, 112, 453-465.
Pollard, T. D. & Cooper, J. A. (2009) Actin, a central player in cell shape and movement. Science,
326, 1208-1212.
Rogers, S. L., Wiedemann, U., Stuurman, N. & Vale, R. D. (2003) Molecular requirements for actin-
based lamella formation in Drosophila S2 cells. J Cell Biol, 162, 1079-1088. Rohatgi, R., Ma, L.,
Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., and Kirschner, M.W. (1999). The interaction
between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.
Cell 97, 221-231.
Rottner, K. & Stradal, T. E. (2011) Actin dynamics and turnover in cell motility. Curr Opin Cell
Biol, 23, 569-578.
Small, J. V., Auinger, S., Nemethova, M., Koestler, S., Goldie, K. N., Hoenger, A. & Resch, G. P. (2008)
Unravelling the structure of the lamellipodium. J Microsc, 231, 479-485.
Stephan, R., Gohl, C., Fleige, A., Klambt, C. & Bogdan, S. (2011) Membrane-targeted WAVE medi-
ates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila. Mol Biol
Cell, 22, 4079-4092.
Tilney, L. G., Tilney, M. S. & Guild, G. M. (1996) Formation of actin flament bundles in the ring
canals of developing Drosophila follicles. J Cell Biol, 133, 61-74.
Vinzenz, M., Nemethova, M., Schur, F., Mueller, J., Narita, A., Urban, E., Winkler, C., Schmeiser,
C., Koestler, S. A., Rottner, K., Resch, G. P., Maeda, Y. & Small, J. V. (2012) Actin branching in the
initiation and maintenance of lamellipodia. J Cell Sci, 125, 2775-2785.
Yan, S., Lv, Z., Winterhoff, M., Wenzl, C., Zobel, T., Faix, J., Bogdan, S. & Grosshans, J. (2013). The
F-BAR protein Cip4/Toca-1 antagonizes the formin Diaphanous in membrane stabilization and
compartmentalization. J Cell Sci. Feb 19
Yang, C. & Svitkina, T. (2011) Visualizing branched actin flaments in lamellipodia by electron
tomography. Nat Cell Biol, 13, 1012-1013; author reply 1013-1014.
Zallen, J. A., Cohen, Y., Hudson, A. M., Cooley, L., Wieschaus, E. & Schejter, E. D. (2002) SCAR is
a primary regulator of Arp2/3-dependent morphological events in Drosophila. J Cell Biol, 156,
689-701.
Zobel, T. & Bogdan, S. A high resolution view of the fy actin cytoskeleton lacking a functional
WAVE complex (2013). J Microsc. Feb 14
Zuchero, J.B., Coutts, A.S., Quinlan, M.E., Thangue, N.B., and Mullins, R.D. (2009). p53-cofactor
JMY is a multifunctional actin nucleation factor. Nat Cell Biol 11, 451-459.¬¬
P H O T O N I S O U R B U S I N E S S
Exceptional quantum efficiency
Over 70
%
at 600nm
High-speed readout
100
frames/s
CameraLink
at 4.0 megapixels
Low Noise
1.3
electrons median
1.9
electrons rms
Standardscan
at 100 frames/s
0.9
electrons median
1.5
electrons rms
Slowscan
at 30 frames/s
DIGITALCAMERA
R
V2
A game changer from inception and a proven performer
since its initial release, the ORCA-Flash4.0 V2 has many new
features and offers unrivalled flexibility across a wide range
of imaging applications.
And then there’s the highest QE of any sCMOS camera
on
the market.
Versatile by Design
1...,14,15,16,17,18,19,20,21,22,23 25,26,27,28,29,30,31,32,33,34,...44
Powered by FlippingBook